SINE's BioLab facility fosters the entrepreneurial venture by providing the high throughput instrumentation for the research and development work. BioLab has a dedicated tissue culture facility to support cancer research, tissue engineering and pharmacological studies.
Facility in-charge | Contact Email | Contact No. | Address | |
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Dr. Pranita Phatak | biolab@sineiitb.org | 02220858595 | SINE BIO-LAB,7th Floor, RBTIC Building, Opp. VMCC, IIT Bombay, Powai, Mumbai – 400 076. |
● Floor model with Max. rotor capacity of 6 × 500 mL.
● Max. speed: 81,770 x g/ 26,000 rpm
● Temperature range: -10° C to 40° C in 1° increments (Temperature Accuracy ±2° C of chamber temperature after equilibration)
● Ambient Operating Range 16° C to 38° C
● Sound Level < 62 dBa (1 m in front of the instrument, 1.5 m above the floor)
● Dimensions 71W x 86D x 86H cm (28W x 34D x 34H in)
● Ergonomic design: Low work surface height for easier installation and unloading of rotors; Foot pedal for hands-free door operation; Door swings up and out of the way for easy access to the rotor chamber; Large digital readouts make operation simpler
● Intuitive interface: User-friendly, large LCD screen; view set and actual run conditions simultaneously; select rotor by name, not by arcane number code; 12 accel/13 decel rates; Delay start; run times to 99:59, Hold, and ω2t integrator for applications that require exquisite reproducibility.
● Safe and sustainable: Friction Reduction System (FRS) allows quicker, quieter, and cooler runs, uses less energy than traditional centrifuges; dynamic rotor inertia check; high-torque, Switched Reluctance (SR) drive cycle shortens cycle times
● Advanced, easy-to-use data management: Perfect for shared labs or GMP environments; on-screen text sensitive “help” screen; Up to 1000 user-defined programs; set up and store numerous protocols; password protection for up to 50 unique user profiles; network multiple instruments; data logging and real-time run graphing.
● Versatile across rotors and applications: Compatible with an extensive library of rotors; fixed-angle and swinging bucket options available; fully compatible with lightweight J-Lite rotors; dynamic Rotor Inertia Check (DRIC) and rotor recognition provide redundant safety checks to assure no rotor can be run above its rated speed; temperature control is optimized ensuring sample quality and avoiding unwanted artifacts.
● Rotors available: J A-10 Fixed Angle Rotor; J A-25.50 Fixed-Angle Rotor; J S-5.3 Swinging-Bucket Rotor
● The highly versatile All Spin rotor (J S-5.3 Swinging-Bucket Rotor) accommodates a wide assortment of labware from 5 mL,15 mL, and 50 mL round-bottom tubes to 15 mL and 50 mL conical tubes, and 500 mL conical bottles. Ideal for sucrose/glycerol gradients, centrifugal filtration, binding studies, clearing debris/large particles, pelleting, and plasma protein precipitates.
Description | JA-10 Fixed Angle Rotor | J A-25.50 Fixed-Angle Rotor | J S-5.3 Swinging-Bucket Rotor |
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Maximum speed | 10 000 RPM | 25,000 RPM | 5300 RPM |
Relative Centrifugal Field at maximum speed | 17 700 × g | 75,6000 X g | Deep-well Plates: 6130 X g
500-mL Conical Bottles: 6870 X g |
Capacity X Number of bottle cavities | 500 mL X 6 | 50 mL X 8 | Number of buckets 4 |
Nominal bottle capacity | 500 mL | 50 mL | 5 mL/15 mL/ 50 mL round-bottom tubes, 15 mL/50 mL conical tubes, and 500 mL conical bottles |
Nominal rotor capacity (mL) | 3000 | 400 | 4x500 (Conical)
24 microplates |
Rotor body and lid material | Aluminium | Aluminium | Aluminium |
Number of buckets | - | - | 4 |
• Cell size (W x L x H) 20 x 26 x 7.5 cm
• Tray sizes (OD) (W x L)15 x 7 cm, 7 x 10 cm
• Base buffer volume: ~650 ml
• Buffer recirculation: No
• Bromophenol Blue migration: ~4.5 cm/hr (at 75 V)
• 1-D vertical gel electrophoresis.
• Runs 1 to 4 mini polyacrylamide gels
• Dimensions (W x L x H): 12 x 16 x 18 cm
• Gel size (W x L): Pre-cast 8.6 x 6.8 cm or Hand cast 8.3 x 7.3 cm
• Glass plate size (W x L): Short plate:10.1 x 7.3 cm and Spacer plate: 10.1 x 8.2 cm
• Total buffer volume: 700 ml for 2 gels and 1,000 ml for 4 gels
• Typical run times for SDS-PAGE: 35–45 min (at 200 V constant)